Global Resource Distribution: Allocation of Actin Building Blocks by Profilin
نویسندگان
چکیده
منابع مشابه
In Vivo Importance of Actin Nucleotide Exchange Catalyzed by Profilin
The actin monomer-binding protein, profilin, influences the dynamics of actin filaments in vitro by suppressing nucleation, enhancing nucleotide exchange on actin, and promoting barbed-end assembly. Profilin may also link signaling pathways to actin cytoskeleton organization by binding to the phosphoinositide PIP(2) and to polyproline stretches on several proteins. Although activities of profil...
متن کاملDepolymerization of actin filaments by profilin. Effects of profilin on capping protein function.
Profilin interacts with the barbed ends of actin filaments and is thought to facilitate in vivo actin polymerization. This conclusion is based primarily on in vitro kinetic experiments using relatively low concentrations of profilin (1-5 microm). However, the cell contains actin regulatory proteins with multiple profilin binding sites that potentially can attract millimolar concentrations of pr...
متن کاملMechanism of regulation of actin polymerization by Physarum profilin
Physarum profilin reduces the rates of nucleation and elongation of F-actin and also reduces the extent of polymerization of actin at the steady state in a concentration-dependent fashion. The apparent critical concentration for polymerization of actin is increased by the addition of profilin. These results can be explained by the idea that Physarum profilin forms a 1:1 complex with G-actin and...
متن کاملReinvestigation of the inhibition of actin polymerization by profilin.
In buffer containing 50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 5 mM imidazole, pH 7.5, 0.1 mM CaCl2, 0.2 mM dithiothreitol, 0.01% NaN3, and 0.2 mM ATP, the KD for the formation of the 1:1 complex between Acanthamoeba actin and Acanthamoeba profilin was about 5 microM. When the actin was modified by addition of a pyrenyl group to cysteine 374, the KD increased to about 40 microM but the critical concent...
متن کاملEvaluation of Updating Methods in Building Blocks Dataset
With the increasing use of spatial data in daily life, the production of this data from diverse information sources with different precision and scales has grown widely. Generating new data requires a great deal of time and money. Therefore, one solution is to reduce costs is to update the old data at different scales using new data (produced on a similar scale). One approach to updating data i...
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ژورنال
عنوان ژورنال: Developmental Cell
سال: 2015
ISSN: 1534-5807
DOI: 10.1016/j.devcel.2014.12.022